Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1988-10-21
1992-01-07
Lilling, Herbert J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435148, 435156, 4351721, 435173, 435244, 435245, 435254, 435255, 435921, 435942, C12Q 104, C12P 726, C12P 722, C12N 138
Patent
active
050791451
ABSTRACT:
Mutations are induced in a microorganism selected from the species Saccharomyces cerevisiae or from the species Candia flareri. The resulting mutants are cultured in the presence of a fermentation inhibitor, such as acetaldehyde, ephedrine or PAC-dione, to form colonies having resistance to the inhibitor. Cells from the colonies are isolated and tested for yield of phenyl acetyl carbinol (PAC) in a fermentation with benzaldehyde and pyruvate. Yeast cells from the colonies that produce elevated levels of PAC are selected for use in subsequent fermentations. PAC is useful as an intermediate in the preparation of 1-ephedrine and d-pseudoephedrine, two well-known medicinal chemicals.
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Hageman Robert V.
Heefner Donald L.
Seely Robert J.
Sullivan Sally A.
Yarus Michael J.
Lilling Herbert J.
Synergen Associates, Inc.
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