Process for preparing macrophages, kits, and compositions for th

Details Process for preparing macrophages, kits, and compositions for th Process for preparing macrophages, kits, and compositions for th

1998-02-18

2000-10-31

Lankford, Jr., Leon B.

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Method of storing cells in a viable state

435372, 435405, 435975, C12N 500

Patent

active

061401226

ABSTRACT:
The invention relates to a process for preparing a composition comprising macrophages, optionally activated, and/or cells derived from monocytes with a strong potential for antigen presentation, said process comprising a stage of culture of monocytes present in the starting composition, this stage being preceding and/or followed by a stage of elimination of all or part of the constituents other than the monocytes present in the starting composition, with the aid of antibodies directed against said constituents, and/or followed by a stage of elutriation. The invention also concerns the compositions of kits for reducing this process to practice.

REFERENCES:
Chokri, M. et al., "Production of Human Macrophages with Potent Antitumor Properties (MAK) by Culture of Monocytes in the Presence of GM-CSF and 1.25-Dihydroxy vitamin D3", Anticancer Research, vol. 12, No. 6B, pp. 2257-2260.
Faradji, A. et al., "Apheresis-Elutriation Program for Adoptive Immunotherapy with Autologous Activated Monocytes in Cancer Patients", The International Journal of Artificial Organs, vol. 14, No. 5, 1991, pp. 304-312.
Lopez, M. et al., "Autologous Lymphocytes Prevent the Death of Monocytes in Culture and Promote as DO GM-CSF IL-3 and M-CSF Their Differentiation into Macrophages", Journal of Immunological Methods, vol. 159, No. 1-2, 1993, pp. 29-38.
E. Helsinki et al., "Tumor-Cytolytic Human Macrophages Cultured As Nonadherent Cells: Potential for the Adoptive Immunotherapy of Cancer", Cancer Detection and Prevention, 1990, pp. 471-481.
R. Andreesen et al., "Primary Cultures of Human Blood-Born Macrophages Grown on Hydrophobic Teflon Membranes", Journal of Immunological Methods, vol. 56, 1983, pp. 295-304.
R. Andreesen et al., "Activation of Human Monocyte-Derived Macrophages Cultured on Teflon: Response to Interferon-y during Terminal Maturation in vitro", Immunobiol., vol. 177, 1988, pp. 186-198.

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