Process for preparing immunogenic complexes and pharmaceutical c

Details Process for preparing immunogenic complexes and pharmaceutical c Process for preparing immunogenic complexes and pharmaceutical c

1987-01-14

1990-02-13

Moskowitz, Margaret

Drug, bio-affecting and body treating compositions

Lymphokine

424 89, 424 92, 424450, 424420, 424418, 424491, 424498, 424499, 514 8, 514 21, 514 42, 514885, A61K 966, A61K 964, A61K 3722, A61K 3939

Patent

active

049005499

ABSTRACT:
The invention relates to a process for preparing immunogenic complexes in which an amphiphatic antigenic protein or peptide in dissolved or solubilized form is contacted with a solution containing a detergent, a sterol, and a glycoside comprising hydrophobic and hydrophilic regions, in at least the critical micelle forming concentration, the detergent is removed, and the immunogenic complex formed is purified. Optionally, the solution with which the antigenic protein or peptide is contacted also contains a phospholipid, preferably phosphatidylethanolamine. The preferred sterol is cholesterol, and preferred glycosides are saponins, espeically Quil A.
The immunogenic complex is useful as a vaccine. Its immunogenic power is higher than that of corresponding micelles formed by aggregation of the antigens, and is also higher than that of the antigens incorporated in liposomes.

REFERENCES:
patent: 4053585 (1977-10-01), Allison et al.
patent: 4196191 (1980-04-01), Almeida et al.
patent: 4201767 (1980-05-01), Fullerton et al.
patent: 4354977 (1982-10-01), Hoskinson et al.
patent: 4356169 (1982-10-01), Simons et al.
patent: 4386066 (1983-05-01), O'Rourke et al.
patent: 4438052 (1984-03-01), Weder et al.
patent: 4439199 (1984-03-01), Amkraut et al.
patent: 4578269 (1986-03-01), Morein
patent: 4806350 (1989-02-01), Gerber
Morein et al., J. Gen. Virol., 64,1557-69, 1983.
Skelly et al., Nature, 290, 51-5, 1981.
Nature, vol. 308, Mar. 29, 1984; pp. 457-460, Chesham, Bucks, GB: B. Morein et al.: "Iscom, a Novel Structure for Antigenic Presentation of Membrane Proteins from Enveloped Viruses", Entirely.
Bull. Off. Int. Epiz., 77, 1289-1295 (1972), K. Dalsgaard.
K. Dalsgaard--Archiv fur die Gesamte Virusforschung, 44, 243-254 (1974).
T. Patt & W. Winkler; Arzneimittelforschung, 10 (4), 273-275 (1960).
R. Vochten et al., J. Pharm. Belg., 42, 213-226 (1968).
Develop. Biol. Standard., 42, 65-69 (1978).
Anal. Biochem., 83, 346-356 (1977).
Blake & Gotschlich; J. Exp. Med., 159, 452-462 (1984).
Infect. Immuno., 40: 369-380 (1983).

Affiliated with

Also associated with

Rating

Process for preparing immunogenic complexes and pharmaceutical c does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Process for preparing immunogenic complexes and pharmaceutical c, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for preparing immunogenic complexes and pharmaceutical c will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1166690