Process for preparing human interleukin 2

Details Process for preparing human interleukin 2 Process for preparing human interleukin 2

1981-04-14

1983-08-30

Tanenholtz, Alvin E.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

435240, 435241, 435948, 435 41, 435 70, 424177, C12P 2100, C12N 500, C12N 502, C12R 191, C12P 100, C12P 2102, A61K 3700

Patent

active

044017561

ABSTRACT:
A process for preparing IL-2 from human malignant cells includes culturing human leukemia or lymphoma cells in vitro in a serum containing medium supplemented with various additives. The culture is stimulated by an optimum concentration of a T cell mitogen to produce a supernate which contains IL-2. After a period of time, the supernate is collected and processed to purify the IL-2. Phorbol myristate acetate may be added to the culture medium to boost production of IL-2.

REFERENCES:
Morgan et al., "Selective in vitro Growth of T Lymphocytes from Normal Human Bone Marrows", 193 Science 1007 (1976).
Ruscetti et al., "Functional and Morphological Characterization of Human T Cells Continuously Grown in vitro", 119 The Journal of Immunology, 131 (1977).
Gillis et al., "Long Term Culture of Tumor-Specific Cytotoxic T Cells", 268 Nature 154 (1977).
Gillis et al., "T-Cell Growth Factor: Parameters of Production and a Quantitative Microassay for Activity", 120 The Journal of Immunology 2027 (1978).
Farrar et al., "Biological Relationship of Thymocyte Mitogenic Factor and Factors Enhancing Humoral and Cell-Mediated Immune Responses", 121 The Journal of Immunology 1353 (1978).
Watson et al., "Biochemical and Biological Characterization of Lymphocyte Regulatory Molecules-I, Purification of a Class of Murine Lymphokines", 150 The Journal of Experimental Medicines 849, (1979).
Gillis et al., "Biochemical Characterization of Lymphocyte Regulatory Molecules-II, Purification of a Class of Rat and Human Lymphokines", 124 The Journal of Immunology, 1954 (1980).
Gillis and Watson J. Exp. Med. 152, 1709 (1980).
Farrar and Fuller-Bonar Fed. Proc. 39, 802 (1980).
Gillis, Smith, Watson J. Immunology 124, 1954 (1980).
Gillis, Scheid, Watson Chem. Abstr. 94, 28660 (1981).
Farrar, Simon, Koopman, Fuller-Bonar J. Immunology 121, 1353 (1978).
Watson, Gillis, Marbrook, Mochizuki, Smith J. Exp. Med. 150, 849 (1979).
Watson, Mochizuki, Gillis, Chem. Abstr. 96, 33000 (1981).

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