Process for preparing human coagulation factor VIII protein comp

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

4352402, 4353201, 530383, 536 235, C12N 510, C12N 1512, C12N 1563, C12N 1579

Patent

active

056934998

ABSTRACT:
A plasmid for expression of human coagulation factor VIII H-chain, a plasmid for expression of human coagulation factor VIII L-chain, an animal cell transformed with either said H-chain expression plasmid or said L-chain expression plasmid or with both thereof, and a process for preparing a human coagulation factor VIII protein complex which comprises forming a transformed animal cell by introducing both said H-chain expression plasmid and said L-chain expression plasmid into said animal cell, culturing said cell to produce the human coagulation factor VIII protein complex in the culture medium and collecting the same. The process of the present invention allows for the production of a safe factor VIII at a high expression level applicable for the production of Factor VIII on an industrial scale.

REFERENCES:
patent: 4980456 (1990-12-01), Scandella et al.
patent: 5004803 (1991-04-01), Kaufman et al.
Robson et al. 1986, Introduction to Protein Engineering, Elsevier, New Yo p. 41.
Fay, P.J. 1988, Archives of Biochem and Biophys. 262(2)525-531.
R.L. Burke et al., "The Functional Domains of Coagulation Factor VIII:C", The Journal of Biological Chemistry, vol. 261, No. 27, 25 Sep. 1986, Baltimore, Maryland, pp. 12574-12578.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Process for preparing human coagulation factor VIII protein comp does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Process for preparing human coagulation factor VIII protein comp, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for preparing human coagulation factor VIII protein comp will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-800439

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.