Process for preparing human coagulation factor VIII protein comp

Details Process for preparing human coagulation factor VIII protein comp Process for preparing human coagulation factor VIII protein comp

1994-07-18

1997-12-02

Jacobson, Dian C.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4352402, 4353201, 530383, 536 235, C12N 510, C12N 1512, C12N 1563, C12N 1579

Patent

active

056934998

ABSTRACT:
A plasmid for expression of human coagulation factor VIII H-chain, a plasmid for expression of human coagulation factor VIII L-chain, an animal cell transformed with either said H-chain expression plasmid or said L-chain expression plasmid or with both thereof, and a process for preparing a human coagulation factor VIII protein complex which comprises forming a transformed animal cell by introducing both said H-chain expression plasmid and said L-chain expression plasmid into said animal cell, culturing said cell to produce the human coagulation factor VIII protein complex in the culture medium and collecting the same. The process of the present invention allows for the production of a safe factor VIII at a high expression level applicable for the production of Factor VIII on an industrial scale.

REFERENCES:
patent: 4980456 (1990-12-01), Scandella et al.
patent: 5004803 (1991-04-01), Kaufman et al.
Robson et al. 1986, Introduction to Protein Engineering, Elsevier, New Yo p. 41.
Fay, P.J. 1988, Archives of Biochem and Biophys. 262(2)525-531.
R.L. Burke et al., "The Functional Domains of Coagulation Factor VIII:C", The Journal of Biological Chemistry, vol. 261, No. 27, 25 Sep. 1986, Baltimore, Maryland, pp. 12574-12578.

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