Process for preparing high purity protein A preparation

Details Process for preparing high purity protein A preparation Process for preparing high purity protein A preparation

1992-02-10

1994-05-24

Furman, Keith C.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

530412, 530416, 530417, 530418, 530419, 530427, C07K 1504, C07K 324, C07K 320, C07K 328

Patent

active

053149939

ABSTRACT:
The invention concerns a process for purifying protein A preparations to high purity with high product yield. Where the protein A is obtained from a Gram-negative recombinant microbe hosting a vehicle containing a gene encoding protein A, the protein A is purified to high purity, and, advantageously, to very low levels of endotoxin. The protein A preparations made via the invention process are useful in therapeutic application, e.g., therapeutic plasma exchange, as well as for other well-known uses of protein A.

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Sofer, G. 1984, Bio/Technology, Dec. pp. 1035-1038.
Chemical Abstract No. 106:137001c, Kudo et al. JP61,224,997.
Chemical Abstract No. 103:176670c. Sehi et al. 1985, Microbiol Immunol, 29(6):559-563.
Lofdahl et al. 1983, Proc. Natl. Acad. Sci. 80:697-701.
Pharmacia, 1986, Separation News vol. 13(6) pp. 2-8, Publication SN50-01-339.
Scoper, R. K. 1982, Protein Purification, Springer-Verlag, N.Y., pp. 52-101.
Schrezenmeier et al. 1987, J. Immunol. Meth. 105:133-137.

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