Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1987-03-19
1989-09-26
Schofer, Joseph L.
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
514 56, 536 551, 536 552, 536 553, 536 54, A61K 3172.5, C08B 3710
Patent
active
048701661
ABSTRACT:
A process for preparing dermatan sulphate (DS) of pharmaceutical purity from animal organs rich in mucopolysaccharides (MPS), by: (a) stabilizing the fresh organs by freezing them either as such or in the form of powder, (b) micronizing the material containing the MPS with an aqueous CaCl.sub.2 solution, (c) digesting the homogenate comprising the raw material and the CaCl.sub.2 with proteolytic enzymes at alkaline pH and at low temperature, (d) acidifying, heating and filtering the lysate, (e) treating the filtrate with quaternary ammonium salts able to undergo complexing with and thus precipitate either the DS along or all the MPS selectively, (f) recovering and purifying the DA either directly from the ammonium salt complex containing it or from the complex obtained from the mixture of ammonium salt complexes of all the MPS by fractional solubilization with acetone.
REFERENCES:
patent: 3862003 (1975-01-01), Okuyama et al.
patent: 4783447 (1988-11-01), Del Bono et al.
Buddecke et al. Hoppe-Seyler's Z. Physiol Chem., 327:49-64.
Dheu-Andries et al. Carbohydrate Research 124:324-322, 1983.
De Ambrosi Luigi
Del Bono Rinaldo
Ferrari Gianni
Pagella Pier G.
Rugarli Pier L.
Kunz Gary L.
Mediolanum Farmaceutici Srl
Schofer Joseph L.
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