Process for preparing hepatitis C virus envelope glycoproteins

Details Process for preparing hepatitis C virus envelope glycoproteins Process for preparing hepatitis C virus envelope glycoproteins

1994-11-04

1999-11-16

Woodward, Michael P.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 5, 435358, 536 2372, C12N 1551, C12Q 170, C07H 2104

Patent

active

059856092

ABSTRACT:
The present invention relates to a novel process for preparing hepatitis C virus (HCV) envelope glycoproteins employing Chinese Hamster Ovary (CHO) cells transformed with recombinant expression vectors containing the hepatitis C virus genome. The present invention provides CHO cells cotransfected with DHFR (dihydrofolate reductase) minigene pDCHIP and recombinant expression vectors containing cDNAs of HCV E1 and E2/NS1 ligated with tissue plasminogen activator signal sequence. HCV E1 and E2/NS1 envelope glycoproteins are produced in a massive manner from the transformed CHO cells adapted in methotrexate. The HCV envelope glycoproteins produced by the present invention can be applied to the development of a diagnostic reagent and a potential preventive HCV vaccine.

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