Process for preparing glucagon or fragments or derivatives there

Details Process for preparing glucagon or fragments or derivatives there Process for preparing glucagon or fragments or derivatives there

1986-01-21

1989-05-02

Teskin, Robin

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

435 70, 435254, 435255, 435256, 435320, 4351723, 935 13, 935 28, 935 48, 935 69, C12P 2100, C12P 2102, C12N 500, C12N 1500

Patent

active

048267635

ABSTRACT:
Glucagon or fragments or derivatives thereof are prepared by cultivation of a yeat strain transformed with a replicable expression vehicle comprising a gene encoding the expression of such products. Synthetic genes encoding glucagon or derivatives thereof have been constructed. Also provided are replicable expression vehicles comprising a replication system for providing stable maintenance in yeast and a DNA-sequence encoding glucagon or fragments or derivatives thereof and transformant yeast strains containing such expression vehicles.

REFERENCES:
patent: 4401759 (1983-08-01), Rubin et al.
patent: 4546082 (1985-10-01), Kurjan et al.
patent: 4598065 (1986-07-01), Lundt
Kurjan et al., Cell vol. 30 1982, pp. 933-943.
Lund et al., PNC Natl. Acad. Sci. Jan. 1982 vol. 79 pp. 345-344 "Pancreatic Preproglucagon cDNA contains two glucagon-related coding Sequences arranged in tandem".
Cabezon et al. PNC Natl. Acad., Sci. vol. 81 pp. 6544-6598 Nov. 1984 "Expression of human .alpha., -antitrypsin cDNA on the yeast Succharomyces cerevisiae".
Ztakura et al., Science vol. 198 vol. No. 56-1063.

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