Process for preparing esters of human insulin

Details Process for preparing esters of human insulin Process for preparing esters of human insulin

1982-08-09

1984-12-18

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Using a micro-organism to make a protein or polypeptide

435213, C12P 2104, C12N 976

Patent

active

044891598

ABSTRACT:
A process for converting human des-B30-insulin, namely ##STR1## into h-In-Thr.sup.B30 esters through amidation with an L-threonine ester in a mixture of water and a water miscible solvent in the presence of trypsin and optionally an acid.
Yields in excess of at least 90% are obtained by limiting water content to 10%-30% v/v of the reaction mixture.

REFERENCES:
patent: 3276961 (1966-10-01), Bodanszky et al.
patent: 3903068 (1975-09-01), Ruttenberg
patent: 4320196 (1982-03-01), Morihara et al.
patent: 4320197 (1982-03-01), Morihara et al.
patent: 4343898 (1982-08-01), Markussen
Butler et al., Arch. Biochem. Biophys., 178, 43-50 (1977).
Morihara et al., Nature, 280, 412-413 (1979).
Obermeier et al., Hoppe-Seyler's Z. Physiol. Chem. 357, 759-767 (1976).
Schmitt et al., Hoppe-Seyler s Z. Physiol. Chem., 359, 799-802 (1978).

Affiliated with

Also associated with

Rating

Process for preparing esters of human insulin does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Process for preparing esters of human insulin, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for preparing esters of human insulin will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1989003