Process for preparing erythritol using novel cell of pichia

Details Process for preparing erythritol using novel cell of pichia Process for preparing erythritol using novel cell of pichia

1998-09-16

1999-12-14

Lilling, Herbert J.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing oxygen-containing organic compound

4352555, 435938, C12N 116, C12P 702

Patent

active

060016167

ABSTRACT:
The present invention relates to a fermentation process to have a high productivity with a novel mutant of Pichia sp., more specifically, for preparing erythritol under optimal fermentation conditions for maximum erythritol production by optimizing the environmental conditions of culture such as pH, temperature and by controlling osmotic pressure. A two-stage fermentation was performed to control osmotic pressure. Osmotic pressure was adjusted to a low level during growth phase and to a relatively high level during production phase by adding continuously glucose and NaCl or KCl. Therefore, erythritol production could be increased.

REFERENCES:
Hajny, et al., Applied Microbiology, 12:3, pp. 240-246, May 1964.
Ishizuka, et al., Journal of Fermentation and Bioengineering, 68:5, pp. 310-314, 1989.
Aoki, et al., Biotechnology Letters, 15:4, pp. 383-388, Apr. 1993.

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