Process for preparing enzyme electrodes

Details Process for preparing enzyme electrodes Process for preparing enzyme electrodes

1988-01-20

1990-11-20

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an...

204403, 435 14, 435 25, 435177, 435288, 435817, C12N 1114, C12N 1102, C12M 140, G01N 2726

Patent

active

049719017

ABSTRACT:
An enzyme electrode is prepared by a process wherein an albumin solution containing 0.05 to 0.5% by weight of a multifunctional aldehyde is applied onto the surface of an electroconductive substrate while maintaining the atmospheric temperature at 0.degree. to 20.degree. C. to thereby form a crosslinked albumin layer on the surface of the electroconductive substrate. Thereafter a solution containing 0.5% by weight or less of a multifunctional aldehyde and a predetermined amount of an oxidase capable of producing hydrogen peroxide (H.sub.2 O.sub.2) is applied on the surface of the previously formed crosslinked albumin layer to form a H.sub.2 O.sub.2 productive oxidase containing layer.

REFERENCES:
patent: 4229536 (1980-10-01), DeFilippi
patent: 4266029 (1981-05-01), Branner-Jorgensen
patent: 4464468 (1984-08-01), Avrameas et al.
patent: 4476005 (1984-10-01), Tokinaga et al.
patent: 4795707 (1989-01-01), Niiyama et al.
patent: 4833075 (1989-05-01), Vijayalakshmi et al.
Vadgama, P., J. Med. Eng. & Technol., vol. 5, No. 6, 1981, pp. 293-298.

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