Process for preparing deacetylcephalosporin C

Details Process for preparing deacetylcephalosporin C Process for preparing deacetylcephalosporin C

1988-10-24

1992-01-21

Robinson, Douglas W.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound having a 1-thia-5-aza-bicyclo

435189, 435231, 435886, 424 946, C12P 3506, C12N 902, C12N 986/1/00

Patent

active

050827723

ABSTRACT:
Deacetoxycephalosporin C synthase obtained from cell-free extracts of Streptomyces clavuligerus was purified to near homogeneity and characterized in inactive form. The amino acid sequence was determined and the synthase recombinantly reproduced in active form in E. coli. The recombinantly produced synthase is provided in about 97% pure form by a chromatographic process. The synthase lacks DAOC hydroxylase function; however, it possesses the ability to transform 3-exomethylenocephalosporin C to deacetylcephalosporin C.

REFERENCES:
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patent: 4536476 (1985-08-01), Wolfe et al.
patent: 4693977 (1987-09-01), Wolfe et al.
Demain et al., Microbial Degradation of Cephalosporin C, Nature, pp. 909 and 910, 1963.
Rollins et al., Purification and Initial Characterization, Canadian Journal of Micro., vol. 34, No. 11, pp. 1196-1202.
Dotzlaf, J. E., and Yeh, W-K., "Copurification and Characterization of Deacetoxycephalosporin C Synthase/Hydroxylase . . .", J. Bacteriol., Apr. 1987, pp. 1611-1618.
Jensen, S. E. et al., "Deacetoxycephalosporin C Synthetase and Desacetoxycephalosporin C Hydroxylase are Two Separate Enzymes in S. clavuligerus", J. Antibiotics 38, 263-265, 1985.
Cortes, J., et al., J. Gen. Microbiol. (1987), 133, 3165-3174.

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