Process for preparing and purifying alpha-interferon

Details Process for preparing and purifying alpha-interferon Process for preparing and purifying alpha-interferon

1991-06-12

1993-03-23

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

530351, 530413, 530416, 530419, 424 854, 424 857, 4352523, C07K 1300, C07K 328, C07K 322, C07K 324

Patent

active

051963230

ABSTRACT:
A process for the preparation and purification of recombinant Interferon-.alpha. is disclosed. The invention is directed to a process comprising the following steps: cultivating E. coli containing the interferon gene for a growth period during which not more than 5% methionine-interferon is formed; extracting and concentrating the expressed interferon; subjecting the preliminarily purified material to Tandem Chromatography, wherein the Tandem Chromatography comprises separation on a cellulose column followed by an anti-alpha-interferon monoclonal antibody affinity column; subjecting the thus purified material to isoelectric precipitation of impurities at about pH 4.0 to about pH 4.8; and purifying the interferon by chromatography on a high performance cation exchange column using a volatile buffer, wherein the purified interferon is non-immunogenic when administered parenterally to a human.

REFERENCES:
patent: 4364863 (1982-12-01), Leibowitz et al.
patent: 4496537 (1985-01-01), Kuan
patent: 4535906 (1985-08-01), Johnston
patent: 4680260 (1987-07-01), Debabov et al.
Hitzman et al. (1983) Science 219, 620-625.
Zwanthoff et al. (1985) J. Gen. Virol. 66, 685-691.
Staehelin et al., J. Bio. Chem. 256:9750-9754 (1981).
Protzman et al., J. Immunol. Methods 75:317-323 (1984).
Wetzel et al., J. Interferon Res. 1:381-390 (1981).
Copy of European Search report for European Application Publication No. 203382.
Antonelli, G. et al., JID:163:882-885 (1991).
Inglada, L. et al., The Lancet, p. 1521, Dec. 26, 1987.
von Wussow, P. et al., The Lancet, pp. 635-636, Sep. 12, 1987.

Affiliated with

Also associated with

Rating

Process for preparing and purifying alpha-interferon does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Process for preparing and purifying alpha-interferon, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for preparing and purifying alpha-interferon will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1350975