Process for preparing a protein which inhibits metastasis of can

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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530412, 530416, 530418, 530422, 530427, 530350, C12P 2102, C07K 1447

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058276912

ABSTRACT:
Disclosed is a novel protein which has a molecular weight of 45,000.+-.5,000 and pI 5.7.+-.0.5 and exhibits cancer metastasis-inhibitory activity. The protein can be prepared by culturing human cells, animal cells and microorganisms capable of producing the protein in a nutrient culture medium while stimulating them with an inducer such as Bacille Calmette-Guerin and lipopolysaccharide.

REFERENCES:
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Cook et al, Cloning, Sequence and Expression Analysis of a Chromosome . . . Cancer, J. Biol. Chem. vol. 268, pp. 17010-17017, 1993.
Y.E. Miller et al, Human Aminoacylase-1: Cloning, Regional Assignment . . . Chromosome 18, Genomics, vol. 8, pp. 149-154, 1990.
Y. Naomoto et al, Journal of Cancer Research and Clinical Oncology, vol. 113, 1987, pp. 544-549.
U.K. Laemmli, Nature, vol. 227, 1970, pp. 680-685.
J. Sambrook et al, Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, 1989, Chapter 8, "Construction and Analysis of cDNA Laboratories", pp. 8.2-8.6.

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