Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1990-06-27
1992-04-28
Rosen, Sam
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435 95, 435101, 435202, C12P 1914
Patent
active
051089130
ABSTRACT:
.alpha.-Amylase is determined by the enzymatic splitting of an .alpha.-amylase substrate and measurement of a fission product, wherein there is used as a substrate a maltoheptaose compound of the formula ##STR1## wherein R is a glucoside, phenylglucoside, mononitrophenylglucoside, dinitrophenylglucoside, sorbitol or gluconic acid group. Reagents comprising such a substrate and a system for the determination of a fission product formed from the amylase substrate by .alpha.-amylase, are also provided. A process for the preparation of a maltoheptaose will Bacillus macerans amylase, free of p-nitrophenyl-alpha-glucoside splitting activity is disclosed.
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Deneke Ulfert
Gruber Wolfgang
Hagen Alexander
Neumann Ulrich
Rauscher Elli
Boehringer Mannheim GmbH
Rosen Sam
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