Process for preparing a human thrombin concentrate intended for

Details Process for preparing a human thrombin concentrate intended for Process for preparing a human thrombin concentrate intended for

1992-07-17

1994-04-19

Robinson, Douglas W.

Drug, bio-affecting and body treating compositions

Enzyme or coenzyme containing

Hydrolases

424530, 530412, 530381, A61K 3514, C07K 300, C07K 1300

Patent

active

053043723

ABSTRACT:
Process for preparing a human thrombin concentrate from the PPSB fraction of plasma that does not involve any addition of factors of animal origin to induce activation of the prothrombin to produce thrombin, and which includes a viral inactivation step using solvent-detergent and purification by cation exchange chromatography. The choice of a protective medium ensures the high specific activity of the final product. The thrombin concentrate obtained using this process is intended for therapeutic use, either alone, to serve as a local hemostatic agent, or in combination with a fibrinogen concentrate to form biological glue.

REFERENCES:
patent: 3819528 (1974-06-01), Berry
patent: 4447416 (1984-05-01), Menache-Aronson et al.
patent: 4627879 (1986-12-01), Rose et al.
patent: 4749783 (1988-06-01), Gordon et al.
patent: 4965203 (1990-10-01), Silbering et al.
patent: 5130244 (1992-07-01), Nishimaki et al.
patent: 5143838 (1992-09-01), Kraus et al.
patent: 5149540 (1992-09-01), Kunihiro et al.
patent: 5151355 (1992-09-01), Crowley et al.
patent: 5151499 (1992-09-01), Kameyama et al.
"Human Thrombins Production, Evaluation, and Properties of -Thrombin*" Journal Biological Chemistry, vol. 262, No. 11, Jun. 10, pp. 3587-3598, 1977.
"Note on the Preparation of Bovine Thrombin" Thrombosis Research 20; pp. 281-283 1980.
"Purification de la Thrombine Humaine Par Chromatographie d'Affinite en vue de son Utilisation Dans les Preparations de Colle Biologique" Manuscript recu le 6.07.89. Accepte le 3.10.89.
"The Purification and Properties of Bovine Thrombin" The Journal of Biological Chemistry, vol. 245, No. 19, Oct. 10, pp. 4857-4862, 1970.
"A Rapid Method For The Purification of Bovine Thrombin & The Inhibition of the Purified Enzyme W/Phenylmethylsulfonyl Fluoride*" Biochemistry vol. 10, No. 13, 1971.
"Purification of Thrombin by Affinity Chromatography on Immobilized Heparin" vol. 11, pp. 799-808, 1977.
"Affinity Chromatography: Purification of Bovine Trypsin and Thrombin.sup.1 " Biochemistry & Biophysics 154, 501-599 (1973).
"Thrombin Purification by One-Step Preparative Affinity Chromatography on Modified Polystyrenes" Journal of Chromatography, 363 (1986) 95-100.
"Large-Scale Preparation and Preliminary Characterization of Human Thrombin" BioChem. Biophys. Acta, 229 (1971) 26-32.
"Reactifs Biochimiques et Organiques" 1990 Lisle d'abreau Chesnes BP. 701, 38297 La Verpilliere Cedex, France PCT/US83/000549 filed on Apr. 14, 1983.
"Purification De La Prothrombine Humaine Par Chromatographie Sur Deae-Sephadex Properties De La Prothrombine Purifiee" 1968, vol. 1.
"Bovine Thrombin and Activated Faxtor X" The Journal of biological Chemistry, vol. 243, No. Jan. 10, pp. 112-117, 1968.
"Bovine Thrombin Purification and Certain Properties" The Journal of Biological Chemistry, vol. 242, No. 22, Nov. 25, pp. 5252-5250, 1967.
"Multiple Bovine Thrombin Components*" The Journal of Biological Chemistry, vol. 245, No. 19, Oct. 10, pp. 5049-5056, 1970.

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