Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1995-01-13
1997-04-08
Jagannathan, Vasu
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4353201, 530399, 530402, 536 2351, C12P 2106, C12N 510, C12N 1518, C07K 1461
Patent
active
056186975
ABSTRACT:
A process for producing ripe human growth hormone is described which comprises introducing into bacteria a recombinant DNA vector comprising a DNA sequence encoding an amino-terminal extended human growth hormone which amino-terminal extension has a negatively charged amino acid sequence and has an even number of amino acids, growing the bacteria such that the amino-terminal extended human growth is expressed, separating the amino-terminal extended human grown hormone from contaminants, cleaving the amino-terminal extension with dipeptidyl aminopeptidase I to obtain ripe human growth hormone and isolating the ripe human growth hormone produced.
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patent: 5424199 (1995-06-01), Goedell et al.
McDonald et al., Barrett (ed) Prot. in Mammalian Cells, pp. 311-322 (1977).
Hill et al., Leucine Aminopeptidase, vol. 2228, pp. 577-599 (1957).
Blumberg et al., Biotech. Gen Corp. pp. A285-292 (1984).
McDonald et al., Tissue Proteinases, pp. 69-107 (1971).
Delange et al., Molec. Biol. Inst., vol. III, pp. 81-118 (1971).
Callahan et al. 1972 Fed. Proc. 31(3):1105.
Goeddel et al. 1979 Nature 281:544.
Christensen Thorkild
Dalb.o slashed.ge Henrik
Jessen Torben E.
Pedersen John
Ringsted J.o slashed.rli W.
Carlson K. Cochrane
Jagannathan Vasu
Novo Nordisk A S
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