Process for preparing a curing agent for myelogenic leukemia

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C12K 900

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041000226

ABSTRACT:
A process for preparing a therapeutic agent characterized by preculturing human amnion cells isolated from human amnion by trypsinization or human amnion cells obtained by subculturing the isolated cells in a medium containing calf or bovine fetal serum at least one time for 1 to 5 days to form a monolayer, removing the medium, adding a medium containing 0.5 to 20 mg of human serum albumin per 1 ml of medium thereto and culturing it for 3 to 6 days, dialyzing the culture liquid against diluted buffer or water, and filtering the dialyzed culture liquid through a bacterial filter thereby obtaining a germ free liquid agent.

REFERENCES:
chem. Abst. Gen. Subj. Index-vol. 8.3 (1975) p. 98 G.S.

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