Process for preparing a carrier useful in immunoassays by deposi

Details Process for preparing a carrier useful in immunoassays by deposi Process for preparing a carrier useful in immunoassays by deposi

1987-11-24

1991-10-29

Rosen, Sam

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

Carrier is inorganic

436518, 436524, 436528, 436530, 436531, 436532, G01N 33552

Patent

active

050616404

ABSTRACT:
The present invention provides a process for the preparation of a specifically bindable protein substance bound to an insoluble carrier material, especially for use in a heterogeneous analysis process, wherein a soluable protein with a molecular weight above about 500,000, which is more hydrophobic than the specifically bindable substance, is coupled to the specifically bindable substance and then the conjugate of reaction component and protein is adsorbed on a hydrophobic solid phase.
The present invention also provides a carrier material for use in solid phase immunoassays including a hydrophobic solid phase which is adsorbed on a conjugate of a hydrophobic protein with a molecular weight above about 500,000 and of a specifically bindable protein or protein containing substance.

REFERENCES:
patent: 3966580 (1976-06-01), Janata et al.
patent: 4006059 (1977-02-01), Butler
patent: 4069352 (1978-01-01), Parsons, Jr.
patent: 4410654 (1983-10-01), Cooper et al.
patent: 4572901 (1986-02-01), Ceriani et al.
patent: 4808530 (1989-02-01), Means et al.
Eiji Ishikawa, et al., Enzyme-Labeling of Antibodies and Their Fragments for Enzyme Immunoassay and Immunohistochemical Staining, Journal of Immunoassay, 4(3), 209-227 (1983).

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