Process for preparing a calcium-dependent oxygenase

Details Process for preparing a calcium-dependent oxygenase Process for preparing a calcium-dependent oxygenase

1988-07-13

1991-06-11

Robinson, Douglas W.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

435 25, C12N 902, C12Q 126

Patent

active

050231812

ABSTRACT:
A process for preparing a Ca-dependent oxygenase having a continuously photogenic performance for a long time when it is continuously added to a substrate such as coelenterazine, which process comprises treating an apoaequorin obtained from natural photoprotein aequorin or an apoaequorin obtained by subjecting an apoaequorin to recombinant DNA technique, with a reducing agent such as 2-mercaptoethanol and reacting the resulting apoaequorin with a metal ion such as calcium ion, preferably in the presence of a substrate coelenterazine.

REFERENCES:
Shimomura et al., Nature 256: 236-238, (1975).
Izutsu et al., Biochem. Biophys. Res. Comm. 49 (4): 1034-1039, (1972).

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