Process for preparing 2-keto-L-gulonic acid

Details Process for preparing 2-keto-L-gulonic acid Process for preparing 2-keto-L-gulonic acid

1985-06-21

1987-09-29

Marantz, Sidney

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Process involving micro-organisms of different genera in the...

435138, C12P 3900, C12P 760

Patent

active

046968970

ABSTRACT:
2-Keto-L-gulonic acid is prepared directly from D-glucose by microbial conversion utilizing mixed culturing on a medium containing D-glucose, employing two kinds of microorganisms; the first, a 2,5-diketo-D-gluconic acid producing microorganism which belongs to genus Erwinia and the second, a 2-keto-L-gulonic acid producing microorganism which belongs to genus Brevibacterium or Corynebacterium. The incubation of the microorganisms in a medium containing D-glucose is used in the disclosed process. By-production of 2-keto-D-gluconic acid, the undesired isomer of the intended product is effectively prevented by employing mixed culturing because of the co-existence of both microorganisms in the medium during at least part of the entire cultivation. Namely, 2-keto-D-gluconic acid produced by the second microorganism is utilized by the first microorganism to produce 2,5-diketo-D-gluconic acid which is subsequently converted into 2-keto-L-gulonic acid by the second microorganism.

REFERENCES:
patent: 3998697 (1976-12-01), Sonoyama
"Identification Methods for Microbiologists", B. M. Gibbs et al., eds., Part B, Chapt. entitled Methods for Identifying Acetic Acid Bacteria, by J. G. Carr, pp. 1-8, Academic Press, New York, 1968.
Wakisaka, "Agr. Biol. Chem.", vol. 28, No. 6, pp. 369-374, (1964).
Wakisaka, "Agr. Biol. Chem.", vol. 28, No. 12, pp. 819-827, (1964).
Breed et al., "Bergey's Manual of Determinative Bacteriology", 7th Ed., pp. 182-189; 349-359, (1957).
Bergey's Manual of Determinative Bacteriology", 8th Ed., pp. 251-253, 332-339.
Sonoyama, T. et al., Applied and Environmental Microbiology, vol. 43, No. 5, 1064-1069, (1982).

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