Organic compounds -- part of the class 532-570 series – Organic compounds – Nitrogen attached directly or indirectly to the purine ring...
Patent
1992-01-16
1993-06-01
Ford, John M.
Organic compounds -- part of the class 532-570 series
Organic compounds
Nitrogen attached directly or indirectly to the purine ring...
544322, C07D23948
Patent
active
052161617
DESCRIPTION:
BRIEF SUMMARY
This invention relates to a process for the preparation of a compound useful as an intermediate in the preparation of pharmaceutical compounds.
The compound 2,5-diamino-4,6-dichloropyrimidine of formula (I): ##STR1## is a useful intermediate in the preparation of antiviral nucleoside analogues, such as those described in EP-A-242482 and 319228 (Beecham Group p.l.c.), and via the process described in EP-A-313289 (Beecham Group p.l.c.).
The literature reference for the preparation of this compound (Temple et. al., J. Org. Chem., 40 (21), 3141, 1975) involves a five step synthesis from 5-nitropyrimidine, giving a poor overall yield. Chlorination of 2,5-diamino-4,6-dihydroxypyrimidine of formula (II): ##STR2## or depicted in the tautomeric form (IIA): ##STR3## with phosphorus oxychloride is described as unsuccessful.
The literature route gives a poor yield of product which renders routes utilizing the intermediate of formula (I) commercially unfavorable.
A high yielding process has now been discovered which uses phosphorus oxychloride in the presence of a quaternary ammonium chloride as the chlorinating agents.
Accordingly, the present invention provides a process for the preparation of a compound of formula (I), as hereinbefore defined, which process comprises chlorinating a compound of formula (II), as hereinbefore defined or an acid addition salt thereof with phosphorus oxychloride and a quaternary ammonium chloride or a weak tertiary amine base hydrochloride.
The reaction is preferably carried out using the quaternary ammonium chloride as solvent, by fusing the reagents at about 100.degree. C. The reaction may also be carried out in a polar inert organic solvent such as acetonitrile, tetrahydrofuran, dioxan, nitromethane, diglyme or dimethoxyethane, preferably acetonitrile.
Examples of ammonium substituents in a quaternary ammonium chloride include C.sub.1-12 alkyl, usually C.sub.1-4 alkyl, or phenyl or benzyl. Preferably a quaternary ammonium chloride is methyltriethylammonium or tetraethylammonium chloride.
Phosphorus oxychloride and a quaternary ammonium chloride are usually present in amounts of from 2-10, preferably from 3-6 molar equivalents of the compound of formula (II).
A tertiary amine weak base is, for example, N,N-dimethylaniline or diethylaniline. The base hydrochloride is usually present in an amount of approximately 2-6 molar equivalents with respect to the compound of formula (II).
The reaction is preferably carried out at an elevated temperature of from 30.degree.-120 C., most preferably under reflux and/or with ultrasonization at around 100.degree. C.
Preferably the reaction is allowed to proceed for a period of greater than 12 hours, usually 24-30 hours.
The above described process has the advantage that it is suitable for large scale production of the compound of formula (I).
The compound of formula (II) is prepared from 5-acetamido-2-amino-4,6-dihydroxypyrimidine, or from other corresponding 5-acyl derivatives by the action of concentrated hydrochloric acid. 5-acetamido-2-amino-4,6-dihydroxypyrimidine is prepared by condensing guanidine carbonate with diethyl 2-acetamidomalonate in ethanol or isopropanol.
The following Examples illustrate the invention. The following Description illustrates the preparation of the intermediate of formula (II).
DESCRIPTION
2,5-Diamino-4,6-dihydroxypyrimidine hydrochloride
##STR4##
5-Acetamido-2-amino-4,6-dihydroxypyrimidine (700 g crude) (ex. guanidinium carbonate, diethyl acetamido malonate and ethanol refluxed 48 hours), concentrated hydrochloric acid (2 liters water), (200 ml), were heated to 70.degree. and kept at 70.degree.-75.degree. for 1 hour, then cooled in an ice bath. The product was filtered, washed with HCl (80 ml conc HCl in water 170 ml), then acetone (1 liter), and air dried at 40.degree. to give the title compound (344.5 g). Found: C 24.53, H 4.33, N 28.9 Cl 17.9; C.sub.4 H.sub.6 N.sub.4 O.sub.2 .multidot.HCl.multidot.H.sub.2 O requires C 24.4, H 4.6, N 28.5, Cl 18.0%. NMR C.sup.13 84.59, 115.24, 157.22 ppm DMSO MS/FAB M+H 143. Va
REFERENCES:
Legraverend, M. et al., "A New Route to 2,5 Diamino-4,6-dichloropyrimidine, A Key Precursor of 9-Substituted Guanines", Synthesis, 587-589 (1990).
C.A. 89:2153472s; Abstract of Temple, C., Jr. et al., "Preparation of 2,5 diamino-4,6-dichloropyrimidine via N-(4,6-dichloro-5-nitropyrimidin-2-yl)acetamide: The Preparation of 2-aminopyrimidine intermediates"; Nucleic Acid Chem. 1978, 1, 47-52.
Beecham Group p.l.c.
Ford John M.
Lentz Edward T.
Stercho Yuriy P.
Suter Stuart R.
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