Chemistry: molecular biology and microbiology – Carrier-bound or immobilized enzyme or microbial cell;... – Enzyme or microbial cell is immobilized on or in an...
Reexamination Certificate
2007-09-11
2007-09-11
Wax, Robert A. (Department: 1652)
Chemistry: molecular biology and microbiology
Carrier-bound or immobilized enzyme or microbial cell;...
Enzyme or microbial cell is immobilized on or in an...
C435S190000, C435S072000
Reexamination Certificate
active
10395878
ABSTRACT:
A process for the preparation of thermostable enzyme glucose oxidase of high Transition temperature (Tm) useful for biological processes of high temperature, said process comprising steps of silanizaton of the activated glass beads by evaporative deposition of Aminopropyl triethoxysilane in acetone, heating the silanated glass beads overnight at about 115° C., treating the heated glass beads with glutaraldehyde in phosphate buffer of pH about 6.0 for one hour each, both with and without vacuum, exposing the treated beads to the enzyme at about 30° C. and for time duration ranging between 3-5 hours with intermittent shaking, reducing the Schiff's base obtained in preceeding step by treating the beads of step (d) with sodium cyanoborohydride, obtaining thermostable enzyme immobilized on the beads, blocking the unoccupied regions of the glass beads with glycine, and a method of using the said thermostable enzymes, and a thermostable enzyme thereof.
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Environmental Protection Agency's (EPA's) Cameo guide on hazardous chemicals under Section 112(r) of the Clean Air Act, http://www.epa.gov/ ceppo/cameo/help/chapte9h.htm, printed on Nov. 14, 2005.
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M.D. Gouda et al., “Stability Studies on Immobilized Glucose Oxidase Using an Amperometric Biosensor—Effect of Protein Based Stabilizing Agents”, Electroanalysis, vol. 13, No. 10, pp. 849-855 (2001).
M.D. Gouda et al., “Enhancement of Operational Stability of an Enzyme Biosensor for Glucose and Sucrose Using Protein Based Stabilizing Agents”, Biosensors and Bioelectronics, vol. 17, pp. 503-507 (2002).
Karanth Naikankatte Ganesh
Kumar Mysore Anantharamaiah
Thakur Munna Singh
Vegesna Renu Sarath Babu
Council of Scientific and Industrial Research
Greenblum & Bernstein P.L.C.
Kosson Rosanne
Wax Robert A.
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