Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1995-06-07
1997-12-30
Lankford, Jr., Leon B.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
424 934, 4242821, 4352521, A01N 6300, A61K 4500, C12N 120, C12P 1906
Patent
active
057029270
ABSTRACT:
A xanthan gum having an improved transmittance is disclosed. Further, the transmittance of the xanthan gum is at least 75% in an 1% by weight aqueous solution. The xanthan gum is prepared by subjecting a strain ATCC 55429 or ATCC 55298 to submerged fermentation conditions to produce a broth containing the gum and debris. The broth is then subjected to heating at a temperature from 45.degree. to 70 .degree. C. for a period of time of at least one half to two hours and at a pH of 9 to 12.5. Two enzyme treatments are performed using an alkaline protease and lysozyme of which the order of their application is irrelevant to the recovery of the xanthan gum. The enzymes are applied at specific temperatures and time periods as well as pH conditions such as a protease is applied at a temperature of 40.degree. to 65 .degree. C. for a period of from 20 minutes to five hours at a pH range of 6 to 10; and the lysozyme is applied at a temperature of 25.degree. to 60 .degree. C. for a period of 20 minutes to five hours at a pH range of 5.5 to 8.0. Both of the enzyme treatments are applied after the broth has been heated in step b) and before the recovery of the xanthan gum.
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Armentrout Richard W.
Homma Taira
Murofushi Kanji
Nagura Shigehiro
Lankford , Jr. Leon B.
Shin-Etsu Bio, Inc.
Shin-Etsu Chemical Co. , Ltd.
Ware Deborah K.
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