Chemistry: molecular biology and microbiology – Process of utilizing an enzyme or micro-organism to destroy... – Resolution of optical isomers or purification of organic...
Reexamination Certificate
2001-06-28
2003-10-28
Marx, Irene (Department: 1651)
Chemistry: molecular biology and microbiology
Process of utilizing an enzyme or micro-organism to destroy...
Resolution of optical isomers or purification of organic...
C435S158000, C435S170000, C435S252100
Reexamination Certificate
active
06638759
ABSTRACT:
BACKGROUND OF THE INVENTION
The present invention relates to a process for preparation of optically active 1,2-diols by cultivating microorganisms belonging to the genus Alcaligenes, which 1,2-diols can be chiral building blocks and their intermediates useful for synthesis of optically active compounds such as pharmaceuticals, agrochemicals, physiological active compounds and ferroelectric liquid crystals.
PRIOR ART
In regard to a chemical method for preparation of optically active 1,2-diols, a method by stereoselective hydrolysis of an epoxide compound such as propylene oxide using Co(III)-salene catalyst and by converting it into a remaining optically active epoxide and a produced optically active diol was already reported by Jacobsen et al. (M. Tokunaga et al., Science, 277, 936-938 (1997)).
However, depending on a substrate, the optical purity of a 1,2-diol compound produced is low and the method needs expensive catalyst and therefore, the method is not economical.
As to an enzymatic method, a process of (R)-1,2-propanediol and (R)-1,2-butanediol, respectively from 1-hydroxy-2-propanone and 1-hydroxy-2-butanone by reduction with glycerol dehydrogenase by Lee and Whitesides (Journal of Organic Chemistry, Vol. 51, pp. 25-36 (1986)) and a process of an optically active 1,2-diol by using an oxidative dehalogenating enzyme (halohydrin dehydro-dehalogenase; abbreviated as HDDase) by Suzuki (Tetrahedron/asymmetry, vol.5, 239-246 (1994), Japanese Patent Publication A 7-1479939) were reported.
Furthermore, as to a method using cells of microorganisms as catalyst, a process of a (S)-1,2-diol by reacting resting cells of microorganisms to a 1-hydroxy-2-ketone compound was known (Japanese Patent Publication A 1-320988). As to a process of a (R)-1,2-diol, an optical resolution method of (R)-1,2,4-butanetriol from racemic 1,2,4-butanetriol was known (Japanese Patent Publication A 6-209781).
However, in these enzymatic methods and methods by resting cells of microorganisms, the enzymes or the resting cells of microorganisms to react with a racemic 1,2-diol have to be separately prepared and then have to be subjected to the optical resolution reaction. Namely, as to the enzymatic reaction, the enzyme has to be extracted from the microorganisms by mechanically disruptive procedure etc. As to the method by the resting cells of microorganisms, the procedure of cultivation of the microorganisms and the procedure of the optical resolution are separately carried out and therefore, it takes extra time and procedure. In addition, when the resolution reaction proceeds with the oxidative- and reductive-degradative reaction, it is necessary to add co-enzyme and an electron acceptor to this reaction system and therefore, in view of the recycle of the reaction the enzymatic process is not efficient.
Thus, it was desired to develop the economical and simple process for preparation of optically active 1,2-diol compounds with highly optical purity.
The present inventors extensively engaged in the development of the process and led to completion of the present invention.
DETAILED DESCRIPTION OF THE INVENTION
The present invention relates to an improved process, namely to an efficiently and practically improved method of the above mentioned process for optically active 1,2-diol compounds by the enzymatic method using halohydrin dehydro-dehalogenase (HDDase)(Tetrahedron/asymmetry, vol. 5, 239-246(1994), Japanese Patent Publication A 7-147993).
Namely based on chemical and enzymatic property of the enzyme (HDDase) participating in the above reaction, by conjugating effective reproduction system of co-enzyme of living microorganisms, an economical and simple process for preparation of optically active 1,2-diol compounds of the formula [2] shown below was developed by the present inventors.
Furthermore, the present inventors found that in case of racemic 1,2,4-butanetriol as a substrate being subjected to the optical resolution by the method of the present invention, (S)-2,4-dihydroxybutyric acid is obtainable together with (R)-1,2,4-butanetriol.
The process of optically active 2,4-dihydroxybutyric acid from racemic 2-hydroxy-&ggr;-butyrolactone by the optical resolution method using lactoinase was already reported (Japanese Patent Publication A 9-308497), but it is the first time to report the production of optically active 2,4-dihydroxybutyric acid together with an optically active 1,2-diol compound.
In regard to the known enzymatic method by HDDase mentioned above, in case of degradative elimination of an optically active 1,2-diol compound from a racemic 1,2-diol compound by the stereoselective oxidative degradation reaction, it is necessary to add expensive nicotinamide adenine dinucleotide (abbreviated as NAD
+
) or an artificial electron acceptor as co-enzyme to the reaction mixture separately.
Therefore, in the enzymatic method by HDDase, the reproduction of co-enzyme becomes rate-determination or reaction controlling factor (Tetrahedron/asymmetry, vol.5, 239-246(1994), J. Ferment. Bioeng., vol. 78, 194-196(1994)) and the method is not practical.
The present invention relates to an economical and practical process for preparation of an optically active 1,2-diol compound [2] shown below which is characterized in recovering said compound remaining by oxidative degradative eliminating the other optically active 1,2-diol compound or converting it into a carboxylic acid from a racemic 1,2-diol compound by repeatedly using NAD
+
produced in the growing microorganisms under the aeration, without addition of NAD
+
or an artificial electron acceptor necessary as co-enzyme to the reaction mixture separately.
As mentioned above, the method of the present invention is fundamentally different from the known enzymatic method by purified HDDase and the known method by resting cells of microorganisms (Tetrahedron/asymmetry, vol. 5, 239-246(1994), J. Ferment. Bioeng., vol. 78, 194-196(1994)).
The present invention relates to a process for preparation of an optically active 1,2-diol compound of the following formula [2]:
wherein R is alkyl group, hydroxy substituted alkyl group, or alkenyl group, which comprises reacting a racemic 1,2-diol compound of the following formula [1]:
wherein R is the same as defined,
with a strain belonging to the genus Alcaligenes which is being cultivated under aeration, and recovering said optically active 1,2-diol compound from said culture broth.
In addition, the present invention also relates to, when racemic 1,2,4-butanetriol is used as a substrate (R is hydroxyethyl group in the formula [2]), a process for preparation of (R)-1,2,4-butanetriol and (S)-2,4-dihydroxybutyric acid by reacting said substrate with a strain belonging to the genus Alcaligenes which is being cultivated under aeration.
This reaction is shown as follows:
DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS
The present inventors developed the economical and practical process for an optically active 1,2-diol compound [2] from a racemic 1,2-diol compound [1] by reacting said racemate with microorganisms belonging to the genus Alcaligenes which are being aerobically cultivated in a nutrient culture medium consisting of peptone or yeast extract as a source of nitrogen and 3-chloropropanediol or gluconic acid as a source of carbon, while making NDA
+
reproduce in the said microorganisms without introducing NDA
+
from outside.
The microorganisms used in the present invention are ones producable halohydrin dehydro-dehalogenase (HDDase), for example Alcaligenes sp. DS-S-7G (FERM BP-3098), Alcaligenes sp. DS-S-8S (FERM BP-3099) and Alcaligenes sp. DS-S-1C (FERM BP-3100) belonging to the genus Alcaligenes, which were isolated from the soil by the present inventors and deposited on Nov. 15, 1989 to the National Institute of Bioscience and Human-Technology Agency of Industrial Science and Technology, Japan under Budapest Treaty with the above accession number, respectively.
The above strains were selected from the soil indexing
Idogaki Hideaki
Nakagawa Atsushi
Suzuki Toshio
Daiso Co. Ltd.
Marx Irene
Wenderoth , Lind & Ponack, L.L.P.
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