Process for preparation of multimeric plasma fibronectin

Details Process for preparation of multimeric plasma fibronectin Process for preparation of multimeric plasma fibronectin

1983-12-28

1987-11-10

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

4352403, 424101, 514 8, 530380, A61K 3702, C07K 1514, C12N 500, C12P 2100

Patent

active

047057513

ABSTRACT:
A method for preparation of multimeric fibronectin. Dimeric fibronectin is treated with guanidine. The dimeric fibronectin so treated is then incubated to allow multimeric fibronectin to form therefrom.

REFERENCES:
Oberley et al.; "The Effect of the Dimeric and Multimeric Forms of Fibronectin on the Adhesion and Growth of Primary Glomerular Cells", Experimental Cell Research 145, 1983, pp. 265-276.
Mosher et al.; "Cross-linking of Collagen and Fibronectin by Factor XIIIa"; J. Biol. Chem., vol. 255, No. 3, Feb. 10, 1980, pp. 1181-1188.
Mosher et al.; "In Vitro Formation of Disulfide-bonded Fibronectin Multimers"; J. Biol. Chem. vol. 258, pp. 6595-6601 (1983).
Smith et al.; "Immunological Identification of Two Sulfhydral-containing Fragments of Human Plasma Fibronectin"; J. Biol. Chem. vol. 257, 5831-5838 (1982).
Morrison and Boyd, "Organic Chemistry", (Allyn and Bacon Eds. 1974) p. 1173.

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