Drug – bio-affecting and body treating compositions – Whole live micro-organism – cell – or virus containing – Genetically modified micro-organism – cell – or virus
Patent
1987-11-10
1988-09-13
Schain, Howard E.
Drug, bio-affecting and body treating compositions
Whole live micro-organism, cell, or virus containing
Genetically modified micro-organism, cell, or virus
4351721, 435253, A61K 3910, C12N 100, C12N 1500
Patent
active
047708751
ABSTRACT:
A process for the preparation of a mutant strain of B. bronchiseptica having chromosomal genetic markers carrying at least temperature-sensitive (grown at 42.degree. C.), nalidixic acid-resistant and heat-labile dermonecrotic toxin producing ability negative markers, suitable for the preparation of live attenuated AR vaccine, which comprises subjecting a strain of B. bronchiseptica to an induced mutation, isolating a mutant having the genetic markers of being at least temperature-sensitive (grown at 42.degree. C.), nalidixic acid-resistant and heat-labile dermonecrotic toxin negative, culturing the isolated strain of B. bronchiseptica on a Bordet-gengou agar plate containing nalidixic acid at 42.degree. C., thereafter picking up an organism morphologically resembling a phase-I organism, repeating the procedures of culturing and picking up, and converting the organisms to a heat-labile dermonecrotic toxin-producing ability negative phase-I organism having a complete capsular antigen.
REFERENCES:
patent: 4016253 (1977-04-01), Switzer et al.
patent: 4225583 (1980-09-01), Switzer et al.
patent: 4456588 (1984-06-01), Shimizu
patent: 4530832 (1985-07-01), Goodnow et al.
"Dermonecrotic Activity of Pasteurella multocida Strains Isolated from Pigs in Japanese Fields", Accepted Dec. 1, 1983, by A. Sawata et al., pp. 141-148.
"Characterization of Dermonecrotic Toxin Produced by Serotype D Stains of Pasteurella Multocida", American Journal of Veterinary Research, vol. 45, No. 11, by T. Nakai et al., pp. 2410-2413.
"Intracellular Locations of Dermonecrotic Toxins in Pasteurella Multocids and in Bordetella Bronchiseptica", American Journal of Veterinary Research, vol. 46, No. 4, by T. Nakai et al., pp. 870-874.
"Properties of Dermonecrotic Toxin Prepared from Sonic Extracts of Bordetella bronchiseptica", Infection and Immunity, vol. 52, No. 4, May 1986, by K. Kume et al., pp. 370-377.
Danbara Hirofumi
Kume Katsumi
Nakai Toyotsugu
Nishizawa Hiroshi
Yoshikawa Takashi
Schain Howard E.
The Kitasato Institute
LandOfFree
Process for preparation of improved mutant strain of Bordetella does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Process for preparation of improved mutant strain of Bordetella , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for preparation of improved mutant strain of Bordetella will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-806071