Process for preparation of human glicentin

Details Process for preparation of human glicentin Process for preparation of human glicentin

1994-12-28

1997-06-24

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

435 697, 530308, C12P 2106, C12P 2102, C07K 14605

Patent

active

056416466

ABSTRACT:
Disclosed is a process for the preparation of native human glicentin, which comprises producing by gene technology a fused protein composed of human glicentin having a peptide moiety connected to the N-terminus of human glicentin and removing the connected peptide moiety from the fused protein, the improvement in which a purification of the fused protein and human glicentin is carried out by utilizing a reversible covalent bond of a cystein residue with the stationary phase of a chromatography.

REFERENCES:
patent: 5126249 (1992-06-01), Becker et al.
patent: 5310876 (1994-05-01), Bayer et al.
patent: 5432156 (1995-07-01), Matsuno et al.
Ford, C.F. et al. (1991) "Fusion tails for the recovery and purification of recombinant proteins" Protein Expr. Purif. 2(2-3):95-107.
Persson, M. et al. (1988) "Enzyme purification by genetically attached polycrysteine and polyphenylalanie affinity tails" Anal. Biochem. 172(2):330-337.
Iwakura M. et al. (1993) "immobilization of dihydrofolate reductase by engineered cysteine residue attached to its C-terminal end" J. Biochem. 114(3):339-343.
"The Enzymes" edited by Paul D. Boyer, pp. 105-111, vol. III, IV Dipeptidyltransferase, Academic Press, NY, 1971.
F.W. Studier et al., Methods in Enzymology, vol. 185, pp. 60-89 (1990).
"Proceedings of the Thirteenth Gut Hormone Conference", edited by Japanese Society of Gut Hormones, 1992, vol. 11, pp. 394-401.
Peptide Chemistry 1992, pp. 158-161, 1993, Tohru Mochizuki, et al., "Total Synthesis of Human Glicentin".

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