Process for preparation of control for use in progesterone recep

Details Process for preparation of control for use in progesterone recep Process for preparation of control for use in progesterone recep

1984-02-21

1988-11-15

Moskowitz, Margaret

Chemistry: analytical and immunological testing

Composition for standardization, calibration, simulation,...

436 15, 436 18, 436 63, 436 64, 436 65, 436503, 436817, 424105, 530387, G01N 33567

Patent

active

047849606

ABSTRACT:
A process is disclosed for producing steroid hormone receptor samples to be utilized as controls during assays of various human tissue for steroid hormones, especially estrogen and progesterone. The process comprises collecting tissue known to include such receptors, adding a buffer solution to the tissue, homogenizing the tissue and buffer solution, centrifuging the homogenized mixture, and thereafter collecting the supernatant. The supernatant which contains the desired receptors is subdivided into suitable control sample size and preferably lyophilized to a flake. A process is described for maximizing the number of receptors found in the samples.

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