Process for preparation of control for use in estrogen receptor

Chemistry: analytical and immunological testing – Composition for standardization – calibration – simulation,...

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436 63, 436503, 436174, 435 1, G01N 3350, G01N 3374

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active

044330561

ABSTRACT:
A process is disclosed for producing steroid hormone receptor samples to be utilized as controls during assays of various human tissue for steroid hormones, especially estrogen. The process comprises collecting tissue known to include such receptors, adding a buffer solution to the tissue, homogenizing the tissue and buffer solution, centrifuging the homogenized mixture, and thereafter collecting the supernatant. The supernatant which contains the desired receptors is subdivided into suitable control sample size and preferably lyophilized to a flake.

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patent: 4254095 (1981-03-01), Fisher
patent: 4293536 (1981-10-01), Jensen et al.
Bojar et al., Cancer, vol. 46, pp. 2770-2774, (1980).
Koenders et al., J. Steroid Biochemistry, vol. 9, pp. 947-950, (1978).
Holt et al., Endocrinology, vol. 108(6), pp. 2308-2315, (6-1981).

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