Process for preparation of control for use in estrogen receptor

Details Process for preparation of control for use in estrogen receptor Process for preparation of control for use in estrogen receptor

1981-09-24

1983-12-27

Padgett, Benjamin R.

Chemistry: analytical and immunological testing

Composition for standardization, calibration, simulation,...

436817, 424105, 435240, C09K 300, G01N 3350

Patent

active

044231512

ABSTRACT:
A process is disclosed for producing steroid hormone receptor samples to be utilized as controls during assays of various human tissue for steroid hormones, especially estrogen. The process comprises collecting tissue known to include such receptors, adding a buffer solution to the tissue, homogenizing the tissue and buffer solution, centrifuging the homogenized mixture, and thereafter collecting the supernatant. The supernatant which contains the desired receptors is subdivided into suitable control sample size and preferably lyophilized to a flake.

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A. J. Koenders, J. Geurts-Moespot, and Th. J. Benraad (authors), From Innisbruck Winter Conference on Biochemistry in Clinical Medicine, "Influence of lyophilization and Subsequent Storage of Target Tissue on Steroid Receptor"-1978, p. 211, Chapter 31.
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