Process for preparation of an adventive embryo of podophyllum

Details Process for preparation of an adventive embryo of podophyllum Process for preparation of an adventive embryo of podophyllum

1990-10-26

1993-07-06

Robinson, Douglas W.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

43524046, 43524048, 43524049, C12N 500, C12N 502

Patent

active

052253430

ABSTRACT:
A process for the preparation of an adventive embryo of a plant belonging to the genus Podophyllum, comprising the steps of preparing cells of a plant belonging to the genus Podophyllum, incubating the cells in a sugar free medium or a medium containing up to 2% by weight sugar, and then incubating the cells in a medium containing more than 2% by weight of sugar to generate an adventive embryo; a process for the production of a podophyllotoxin compound comprising the steps of preparing an adventive embryo of a plant belonging to the genus Podophyllum, culturing the adventive embryo to generate an adventitious organ, culturing the adventitious organ to produce a podophyllotoxin compound, and recovering the podophyllotoxin compound; and a process for the production of a podophyllotoxin compound, comprising the steps of preparing de-differentiated cells or tissue of a plant belonging to the genus Podophyllum culturing the de-differentiated cells or tissue in a liquid medium to generate a differentiated organ, culturing the differentiated organ on a solid medium to produce a podophyllotoxin compound, and recovering the podophyllotoxin compound.

REFERENCES:
patent: 4038778 (1977-08-01), Kadkade
Arumugan et al., Can. J. Bot., 68(3), 1990, pp. 487-491 (Biosis Abstract #90000042).

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