Liquid purification or separation – Processes – Liquid/liquid solvent or colloidal extraction or diffusing...
Patent
1999-06-02
2000-12-05
Therkorn, Ernest G.
Liquid purification or separation
Processes
Liquid/liquid solvent or colloidal extraction or diffusing...
210656, 210659, 435 6, 536 254, B01D 1508
Patent
active
061562068
ABSTRACT:
The invention recognizes the deleterious effects of trace, and even undetectable amounts of multivalent cations on the separation of mixtures of polynucleotides, especially double stranded polynucleotides, and provides an improved method for separating such mixtures on wide pore, non-polar separation media by eliminating multivalent cations from the all aspects of the separation process. This is accomplished by using components in the separation process which are materials which do not release metal cations. In addition, the use of cation capture resins and other methods to remove residual traces of multivalent cations from eluting solvents, sample solutions, separation media, and system components is described. It is also important to remove any traces or organic contaminants from solvents solutions and system parts. Taking similar steps to remove residual traces of multivalent cations and organic impurities from the separation process, the invention may also be used in a batch process to separate mixtures of polynucleotide fragments.
REFERENCES:
patent: 4563510 (1986-01-01), Ugelstad
patent: 4683202 (1987-07-01), Mullis
patent: 4855225 (1989-08-01), Fung et al.
patent: 5205929 (1993-04-01), Carr et al.
patent: 5207914 (1993-05-01), Lin
patent: 5338448 (1994-08-01), Gjerde
patent: 5453185 (1995-09-01), Frechet et al.
patent: 5585236 (1996-12-01), Bonn et al.
patent: 5616701 (1997-04-01), Woodard et al.
patent: 5772889 (1998-06-01), Gjerde et al.
patent: 5795976 (1998-08-01), Oefner et al.
patent: 5843312 (1998-12-01), Manz
Barder et al. Fast Chromatography and Nonporous Silica, LC-GC (1997) 15: 918-926, No. 10.
Chen et al. High-Speed High-Performance Liquid Chromatography of Peptides and Proteins, J. of Chromatography A (1995) 705: 3-20.
Huber et al, High-Resolution Liquid Chromatography of DNA Fragments on Non-Porous Poly9styrene-Divinylbenzene) Particles, Nucleic Acid Research, vol. 21 No. 5: 1061-1066 (1993).
Engelhardt et al. Polymer Encapsulated Stationary Phases: Advantages, Properties and Selectivities, Chromatographia (1989) 27: 535-543, No. 11/12.
Erikkson et al, Separation of DNA Restriction Fragments by Ion-Pair Chromatography, Journal of Chromatography, 359: 265-274 (1986).
Huber et al., Micropellicular Stationary Phases for High-Performance Liquid Chromatography of Double-Stranded DNA, J. of Chromatography A 806 (1998) pp. 1-28.
Iler et al. The Chemistry of Silica (1979) John Wiley & Sons, New York pp. 566-569.
Kalghatgi et al. Rapid Analysis of Proteins an Peptides by Reversed-Phase Chromatography, J. of Chromatography (1987) 398:335-339.
Nahum et al. Surface Silnols in Silica-Bonded Huydrocarbonaceous Stationary Phases, J. of Chromatography (1981) 203: 53-63.
Poole et al. Chromatography Today (1991) Elsevier, New York, pp. 313-342.
Schoburg et al. Immobilization of Stationary Liquids in Reversed- and Normal-Phase Liquid Chromatography, J. of Chromatography (1983) 282: 27-39.
Schoburg et al. Immobilization of Stationary Liquids of Silica Particles by Y-Radiation, Chromatographia (1984) 18: 265-274, No. 5.
Stober et al. Controlled Growth of Monodisperse Silica Spheres in the Micron Size Range, (1968) J. of Coll. and Interface Science 26: 62-69.
Wheals, Chemically Bonded Phases for Liquid Chromatography, J. of Chromatography (1975) 107: 402-407.
Apffel et al, 17th Intl. Symposium on the Separation of Proteins, Peptides & Polynucleotides, (Oct. 26-29, 1997).
Karin Cabrera et al., Silica ROD--A New Challenge in Fast High-Performance Liquid Chromatography Separations, Trends in Analytical Chemistry, vol. 17, No. 1, 1998, pp. 50-53.
Heftmann, Chromatography, 5th Edition Journal of Chromatography Library--vol. 51A pp. A299-A300, (1992).
Herold et al, BioChromatography, 10:5 656-662, 1991.
Huber et al., High-Resolution Liquid Chromatography od DNA Fragments on Non-Porous Poly(Styrene-Divinylbenzene) Particles, Nucleic Acids Research, vol. 21, No. 5 1061-1066.
Jinno et al., Planarity Recognition of Large Polycyclic Aromatic Hydrocarbons by Various Octadecylsilica Stationary Phases in Non-Aqueous Reverse-Phase Liquid Chrmatography Apr. 1989 pp. 285-291.
Nakanishi et al. Phase Separtion in Silica Sol-Gel System Containing Poly(ethylene oxide) I. Phase Relation and Gel Morphology, Bull Chem. Soc. Jpn., 67 1327-1335 (1994).
Nakanishi et al., Double Pore Silica Gel Monolith Applied to Liquid Chromatography, J. Sol-Gel Scince and Technology, vol. 8, 547-552 (1997).
Petro et al., Molded Monolithic Rod of Macroporous Poly(styrene-co-divinylbenzene) as a Separation Medium of HPLC . . . Anal. Chem. 1996, 68 315-321.
J. W. Goodwin et al., Colloid & Polymer Sci., 252:464-471 (1974).
J. Hirabayashi et al., Anal. Biochem., 178:336-341 (1989).
J. Hirabayashi et al., Biochemistry, 29:9515-9521 (1990).
Huber et al., Chromatographia, 37:653-658 (1993).
Y. Kato et al., J. Chromatogr., 478:264-268 (1989).
Y. Ohimya et al., Anal. Biochem., 189:126-130 (1990).
R. K. Saiki et al., Science, 230:1350-1354 (1985).
J. Ugelstad et al., Adv. Colloid Interface Sci., 13:101-140 (1980).
All-Chrom Newsletter, 25:1-6 (Jun. 1986).
Gjerde Douglas T.
Haefele Robert M.
Togami David W.
Therkorn Ernest G.
Transgenomic Inc.
Walker William B.
LandOfFree
Process for performing polynucleotide separations does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Process for performing polynucleotide separations, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for performing polynucleotide separations will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-958267