Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1994-03-09
1995-07-25
Parr, Margaret
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435 6, C12Q 168, C12P 1434
Patent
active
054361443
ABSTRACT:
A process is provided for performing polymerase chain reactions inside of intact cells. Measurement of genetic parameters and observation of genetic properties while maintaining the integrity of the DNA or RNA in a cell is accomplished by passing a suspension of cells through a flow cytometer wherein the properties and parameters can be measured on a cell by cell basis.
REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis
patent: 4889818 (1989-12-01), Gelfand et al.
Timm, E. A. et al. Genotyping cells by flow cytometry using PCR techniques, Abstracts for the XIV International Meeting of the Society for Analytical Cytology, Mar. 18-23, 1990, Cytometry (Suppl. 4) p. 79.
Ramphal, R. et al. Pseudomonas aeruginosa Recognizes Carbohydrate Chains Containing Type 1 . . . Infect. Immun. (Feb. 1991) 59:700-704.
Harlow, E. et al. Antibodies: A Laboratory Manual (1988) p. 179.
Spann, W. et al.,-In Situ Amplification of Single Copy Gene Segments in Individual Cells by the Polymerase Chain Reaction, Infection 19, No. 4, 46-48 (1991).
Lo, Y-M. D. et al., Rapid Production of Vector-Free Biotinylated Probes Using the Polymerase Chain Reaction, Nucleic Acids Research 16:8719, (1988).
Feorino, P. M. et al., Lymphadenopathy Associated Virus Infection of a Blood Donor-Recipient Pair with Acquired Immunodeficiency Syndrome, Science 225:69-72 (1984).
Coombs, R. W. et al., Plasmia Viremia in Human Immunodeficiency, NEJM 321:1626-1631 (1989).
Saiki, R. K. et al., Primer Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase, Science 239:487 (1988).
Schriever, F. et al., Isolated Human Follicular Dendritic Cells Display a Unique Antigenic Phenotype, J. Exp. Med. 169:2043-2058 (1989).
Frye, R. A. et al., Detection of Amplified Oncogenes by Differential PCR, Oncogene 4:1153-1157 (1989).
Saiki, R. K. et al., Analysis of Enzymatically Amplified B-globin and HLA-DO.alpha.DNA with Allele-Specific Oligonucleotide Probes, Nature 324:163-165 (1986).
Almoguera, C. et al., Most Human Carcinomas of the Exocrine Pancreas Contain Mutant c-K-ras Genes, Cell:549-554 (1988).
Bauman, J. G. J. et al., Flow Cytometry of Fluorescent In Situ Hybridization to Detect Specific RNA and DNA Sequences, Acta Histochemica Suppl. 37:65-69 (1989).
Singer, R. H. et al., Optimization of In Situ Hybridization Using Isotopic and Non-Isotopic Detection Methods, Biotechniques 4:230 (1986).
Terott, L. H. et al., In Situ Transcription: Specific Synthesis of Complementary DNA in Fixed Tissue Sections, Science 240:1661-1664 (1988).
Kawasaki, E. S. et al., Diagnosis of Chronic Myeloid and Acute Lymphocytic Leukemias by Detection of Leukemia-Specific mRNA Sequences Amplified In Vitro., Proc. Nat'l. Acad. Sci. USA 85:5698-5702 (1988).
Singer, R. H. et al., Actin Gene Expression Visualized in Chicken Muscle Tissue Culture by Using In Situ Hybridization with a Biotinylated Nucleotide Analog, Proc. Nat'l. Acad. Sci. USA 79:7331-7335 (1982).
Mullis, K. B. et al., Specific Synthesis of DNA In VItro Via a Polymerase-Catalyzed Chain Reaction, Methods in Enzymology 155:335-351 (1987).
Pinkel, D. et al., Cytogenetic Analysis Using Quantitative High Sensitivity, Fluorescence Hybridization, Proc. Nat'l. Acad. Sci. USA 83:2934-2938 (1986).
Bakkus, M. et al., Detection of Oncogene Expression by Fluorescent In Situ Hybridization in Combination with Immunofluorescent Staining of Cell Surface Markers, Oncogene 4:1255-1262 (1989).
Haase, A. T., et al., Amplification and Detection of Lentiviral DNA Inside Cells, Proc. Nat'l. Acad. Sci. USA, 87:4971-4975 (1990).
Willman, C. L., et al., Differential Expression and Regulation of the c-src and c-for Protooncogenes in Myelomonocytic Cells, Proc. Nat'l. Acad. Sci. USA 84:4480-4484 (1987).
Stewart Carleton
Timm, Jr. Earl A.
Dunn Michael L.
Health Research , Inc.
Parr Margaret
Schreiber David
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