Plant husbandry – Process
Patent
1997-06-24
1999-08-31
Lankford, Jr., Leon B.
Plant husbandry
Process
47 576, 435420, 435430, 4354301, 435395, 435398, C12N 500
Patent
active
059438215
ABSTRACT:
Plant tissues which have been cultured in vitro are packaged with a nutritive medium for survival of the tissues in a sealed sterile container and with support members so that the plant tissues are positioned between surfaces of the support members. The support members have opposing surfaces for supporting the tissues and for support by a surface of the container and are made of a material suitable for being impregnated by the nutritive medium, and the container has a surface sized suitably for supporting the surface of the support member. At the time of packaging, at least one of the two support members is impregnated with the nutritive medium in an amount so that the tissues attach, by capillarity, to one surface of the at least one impregnated support member and so that the opposing surface of the at least one impregnated support member, when packaged, attaches, by capillarity, to the container surface.
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Delvallse, et al., "Cryopreservation of Immature Maize Embryos After Freeze-Hardening in the Ear and In Vitro", Plant Science, 60, (1989) 129-136.
Chen, et al., "Cryopreservation of Alkaloid-Producing Cell Cultures of Periwinkle (Calharanthus roseus)", Plant Physiol., (1984) 75, 726-731.
Caplin, "Mineral Oil Overlay For Conservation of Plant Tissue Cultures", American Journal of Botany, vol. 46, pp. 324-329.
Derwent Information Ltd., Database Abstract WPI Accession No. 89-364815/198950, abstract of Mark, German Patent Application Publication No. DE 3 818 440 A1.
Agricell Report, Germplasm Exchange Using Alginate-Coated Nodal Segments of Yam, Jan. 1996.
Ducos Jean-Paul
Florin Bruno
Petiard Vincent
Lankford , Jr. Leon B.
Nestec S.A.
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