Process for oligonucleotide synthesis

Details Process for oligonucleotide synthesis Process for oligonucleotide synthesis

1998-10-06

2000-05-30

Tsang, Cecilia

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C07H 2100, C07H 2102, C07H 2104

Patent

active

06069243&

ABSTRACT:
The present invention describes methods for linking phosphoramidite nucleosides to a solid support-bound nucleoside. This invention presents novel processes for synthesizing oligonucleotides. Acetonitrile having a water content between 30 ppm and about 1250 ppm is used as the solvent for the washing steps in solid phase oligonucleotide synthesis, or is used as a solvent for the capping reagent or oxidizing reagent. The processes of the present invention are amenable to large-scale, economic oligonucleotide synthesis using the phosphoramidite method.

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