Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound
Patent
1991-05-28
1993-03-02
Lilling, Herbert J.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing oxygen-containing organic compound
435135, 435136, 435146, 435158, 435176, 435177, 435178, 435179, 435182, 4352536, 435822, C12P 758, C12P 718, C12N 1104, C12N 138
Patent
active
051908697
ABSTRACT:
The long-term activity of the biocatalyst for the production of sorbitol and gluconic acid from an aqueous solution of fructose and glucose is considerably improved with the aid of permeabilized cells of Zymomonas mobilis immobilized using .kappa.-carrageenan that has been rigidified and then stabilized by K.sup.+ ions. Rigidification of .kappa.-carrageenan is preferably carried out by treatment with glutaraldehyde alone or by treatment with polyethyleneimine or hexamethylenediamine and subsequent exposure to glutaraldehyde. A buffer-free solution is preferred and the pH is maintained by adding Ca.sup.++ ions while simultaneously precipitating gluconic acid.
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Bomar, "A Simple Procedure To Immobilize Microbial Cells", pp. 424-426.
Rehr Bert
Sahm Hermann
Forschungszentrum Juelich GmbH
Lilling Herbert J.
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