Process for obtaining sorbitol and gluconic acid or gluconate

Details Process for obtaining sorbitol and gluconic acid or gluconate Process for obtaining sorbitol and gluconic acid or gluconate

1990-10-31

1992-04-07

Lilling, Herbert J.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing oxygen-containing organic compound

435158, 435176, 435177, 435182, 435244, 4352536, 435259, 435822, C12P 718, C12P 758, C12N 120, C12N 138

Patent

active

051027950

ABSTRACT:
A process for obtaining sorbitol and gluconic acid or gluconate from aqueous glucose/fructose mixtures by using Zymomonas mobilis cells which have been permeabilized by treatment with cationic surfactant is disclosed. The permeabilized cells are preferably obtained by treatment with long-chain quaternary alkylammonium salts such as, in particular, CTAB, Dodigen or Bardac. Surfactant concentrations of 0.1 to 0.3% and a treatment time of 1 to 10 minutes at room temperature are expedient. The cell concentrations preferably used for the fermentation are 20 to 80 g/1 dry matter of permeabilized cells.

REFERENCES:
patent: 4775467 (1988-07-01), Scopes et al.
patent: 5017485 (1991-05-01), Bringer-Meyer et al.
H. Felix, "Analytical Biochemistry", Permeabilized Cells, 1982, pp. 211-234.
Reiff et al., "Yeasts in Science", Yeasts, vol. I, 1960, pp. 301-302.

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