Process for obtaining sorbitol and gluconic acid by fermentation

Details Process for obtaining sorbitol and gluconic acid by fermentation Process for obtaining sorbitol and gluconic acid by fermentation

1989-12-11

1991-05-21

Lilling, Herbert J.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing oxygen-containing organic compound

435137, 435146, 4352521, 435259, 435822, C12P 758, C12P 718, C07C 3126, C07C 59105

Patent

active

050174851

ABSTRACT:
A process is disclosed for obtaining sorbitol and gluconic acid, or gluconate, by fermentation in aqueous glucose/fructose mixtures. Zymomonas mobilis cells are used which have been permeabilized by the freeze technique and which have preferably been obtained by freezing cell centrifugates of pH 6 to 7 at about -20.degree. C., and thawing at room temperature. Cell concentrations of 20 to 60 g of cell dry matter of permeabilized cells/l are preferably used for the fermentation. By using cells which have been permeabilized by freezing and thawing, a considerably higher conversion rate of glucose/fructose into sorbitol and gluconic acid, or gluconate, is achieved.

REFERENCES:
patent: 4755467 (1988-07-01), Scopes et al.
Felix, "Permeabilized Cells," Analytical Biochemistry 120, 1982, pp. 211-234.

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