Process for obtaining proinsulin possessing correctly linked cys

Details Process for obtaining proinsulin possessing correctly linked cys Process for obtaining proinsulin possessing correctly linked cys

1993-12-01

1995-12-05

Warden, Jill

Chemistry: natural resins or derivatives; peptides or proteins;

Peptides of 3 to 100 amino acid residues

Insulin; related peptides

530324, A61K 3828, C07K 1462

Patent

active

054730490

ABSTRACT:
A process is described for obtaining proinsulin possessing correctly linked cystine bridges from a corresponding proinsulin amino acid chain in the presence of mercaptan and chaotropic auxiliary agents and at a pH of 10 to 11, as well as its direct isolation from the reaction mixture using hydrophobic adsorber resins.

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patent: 5227293 (1993-07-01), Stengelin et al.
Lowe, P., Rhind, S. K., Sugrue, R., Marston, F. A. O. (1987) Protein Purification, Alan Liss Pub., pp. 429-442.
Biochemistry, 60, (1968), S. 622-629.
Jaenicke, R. Rudolph, 1989, "Folding Proteins", Protein Structure a practical Approach; ed. Creighton, T.E.S. 191-224, JRL Press Oxford.
Kemmler et al., J. Biol. Chem., 246 (1971), S. 6786.
Chem. Abstract 114:99871s.

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