Process for obtaining mammalian insulin secreting cells in...

Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Primate cell – per se

Reexamination Certificate

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C435S325000, C435S378000, C435S379000, C435S380000, C435S381000

Reexamination Certificate

active

06900051

ABSTRACT:
The present invention relates to a process for obtaining mammalian insulin secreting cells in vitro, characterized in that it contains the following steps: a) preparation of the mammalian pancreatic tissues by removal of a pancreas, b) dissociation of the pancreatic tissues obtained in step (a) into isolated pancreatic cells, c) possibly the elimination of the endocrine cells from the pancreatic cells isolated in step (b), d) induction of dedifferentiation of the cells isolated in step (b) into ductal precursor cells, e) induction of redifferentiation of the ductal precursor cells obtained in step (d) into insulin secreting cells. It also concerns the use of the insulin secreting cells thus obtained for the preparation of a pharmaceutical composition which can be used for the treatment of pancreatic pathologies and particularly diabetes.

REFERENCES:
patent: WO 00/46351 (2000-08-01), None
patent: WO 00/78929 (2000-12-01), None
Gmyr, et al., “Adult human cytokeratin 19-positive cells reexpress insulin promoter factor 1 in vitro”, Diabetes 49 (2000) pp. 1671-1680.
Gmyr, et al., “Discarded exocrine tissue may be a potential source of human pancreatic precursors”, Acta Diabetologica 37 (2000) p. 153.
Bonner-Weir, et al., “In vitro cultivation of human islets from expanded ductal tissue”, Proceedings of the National Academy of Sciences of the United States 97 (2000) pp. 7999-8004.
Gmyr, et al., “Expansion of human ductal pancreatic stem cells obtained from main duct, purified islet preparation, or exocrine tissue”, Acta Diabetologica 34 (1997) p. 107.
Kerr-Conte, et al., “Model for islet cell neogenesis from adult human pancreatic ductal epithelium in vitro”, Experimental and Clinical Endocrinology & Diabetes 105 (1997) pp. A28-A29.
Gmyr, et al., “Human pancreatic ductal cells: Large-scale isolation and expansion”, Cell Transplantation 10 (2001) pp. 109-121.

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