Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving transferase
Patent
1978-10-20
1980-12-02
Kepplinger, Esther M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving transferase
435 22, 435 26, 435194, C12N 912, C12Q 132, C12Q 140, C12Q 148
Patent
active
042372216
ABSTRACT:
The present invention provides a process for obtaining maltose phosphorylase and/or .beta.-phosphoglucomutase from micro-organisms, wherein the starting material used is selected from Lactobacillus brevis DSM 20054, NCIB 8836, 8561 and 8562, Lactobacillus plantarum DSM 20174 and 43, Lactobacillus reuteri DSM 20016, Lactobacillus fermentum DSM 20052, Streptococcus spec. DSM 1118, DSM 119, DSM 1120 and DSM 1121.
The present invention also provides a composition and process for determining .alpha.-amylase, wherein maltose phosphorylase and .beta.-phosphoglucomutase obtained by such a process is used, as a crude extract or in an enriched form, optionally with the addition of .alpha.-glucose-1,6-diphosphate and of divalent manganese ions.
REFERENCES:
patent: 4036697 (1977-07-01), Pierre et al.
patent: 4162194 (1979-07-01), Pierre et al.
Kamogawa et al., Agr. Biol. Chem., 37(12), (1973), 2813-2819.
Wood et al., Biochem. J., 78, (1961), 204-209.
Kamogawa et al., Anal. Biochem., 57, (1974), 303-305.
Gauhl Helmgard
Seidel Hans
Boehringer Mannheim GmbH
Kepplinger Esther M.
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