Process for obtaining lipid envelope virus sub-units, notably an

Details Process for obtaining lipid envelope virus sub-units, notably an Process for obtaining lipid envelope virus sub-units, notably an

1984-08-01

1985-06-11

Rose, Shep K.

Drug, bio-affecting and body treating compositions

Lymphokine

435235, 435236, 435238, 435239, A61K 39145

Patent

active

045228094

ABSTRACT:
A process is disclosed for producing virus sub-units with lipid envelopes, comprising, in a neutral or basic pH, dissolving a lower halogenated hydrocarbon, at a concentration equivalent to or approximating its limit of solubility, in an aqueous virus suspension, thereafter contacting, with stirring, the preparation so obtained with a nonionic detergent at a minimum concentration effective to induce disruption of virions into heavy sub-units for a length of time sufficient to permit said disruption, and in separating the heavy sub-units so obtained from the reaction medium. The process permits preparation of purified disrupted antigens for use as vaccines, notably influenza vaccines.

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