Process for obtaining insulin having correctly linked cystine br

Details Process for obtaining insulin having correctly linked cystine br Process for obtaining insulin having correctly linked cystine br

1995-02-16

1997-09-02

Russel, Jeffrey E.

Chemistry: natural resins or derivatives; peptides or proteins;

Peptides of 3 to 100 amino acid residues

Insulin; related peptides

435 681, 530305, C07K 1107, C07K 1400, C12P 2706

Patent

active

056632916

ABSTRACT:
A process for obtaining insulin having correctly linked cystine bridges from a corresponding proinsulin amino acid chain in the presence of mercaptan, chaotropic auxiliaries and at a pH of 10 to 11 is described, as well as the direct conversion of the proinsulin to insulin with the aid of enzymes and direct isolation of the insulin from the reaction mixture with the aid of hydrophobic adsorber resins.

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B. H. Frank et al., "The Production of Human Proinsulin and Its Transformation to Human Insulin and C-peptide," Proc. 7th Am. Pept. Symp., (1981) pp. 729-738.
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