Process for obtaining hybrid strains of yeasts

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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435924, 435248, 435249, 435259, 435267, C12K 102

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041727649

ABSTRACT:
The cell walls are removed from two distinct auxotropic mutant strains of yeast. The protoplasts thus obtained are fused and the cell walls regenerated. The strains obtained are cultured on a medium where the original auxotrophs cannot develop and the hybrid or recombinant strains are recovered. The strains obtained by the process are useful for preparing yeast proteins.

REFERENCES:
patent: 3855063 (1974-12-01), Nagasawa et al.
Ferenczy et al., Nature, vol. 248, pp. 793 & 794 (1974).
Ferenczy et al., Experientia 31/1, pp. 50-52 (1975).
Ferenczy et al., Experientia 31/9, pp. 1028-1030.
Fodor et al., Proc. Natl. Acad. Sci. U.S.A., vol. 73, No. 6, pp. 2147-2150, Jun. 1976.

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