Process for obtaining hepatitis-safe, sterile hemoglobin solutio

Details Process for obtaining hepatitis-safe, sterile hemoglobin solutio Process for obtaining hepatitis-safe, sterile hemoglobin solutio

1982-07-30

1984-03-27

Schain, Howard E.

Chemistry of carbon compounds

Miscellaneous organic carbon compounds

C-metal

2601125R, 424101, 424177, A61K 3518, A61K 3702, C07G 700, C07C10352

Patent

active

044393571

ABSTRACT:
A process for obtaining hepatitis-safe, sterile, pyrogen- and stroma-free hemoglobin solutions of low potassium content comprising stirring an erythrocyte concentrate with about 1 to 6-times its volume of an about 5 to 15% aqueous solution of a sugar, sugar alcohol or high-molecular-weight colloid, adjusting the pH of the resulting suspension to about 5 to 6.5 with an acid which is physiologically tolerable as salt, allowing the erythrocytes to settle, removing the supernatant from the sediments, adjusting the pH of the sediment to about 7 to 8, homogenizing the sediment, adjusting its hematocrit to about 55 to 60%, treating the mass with a dilute beta-propiolactone solution, hemolyzing the residual solids and forming a suspension thereof, contacting the suspension with a cation exchanger in H.sup.+ form until the pH has decreased to about 5 to 5.5 thereby to precipitate the stroma, separating from the liquid the cation exchanger and precipitated stroma, and adjusting the pH to about 7.2 to 7.6.

REFERENCES:
patent: 3864478 (1975-02-01), Bonhard
patent: 4001200 (1977-01-01), Bonsen et al.
patent: 4136093 (1979-01-01), Bonhard et al.
patent: 4336248 (1982-06-01), Bonhard et al.
patent: 4376727 (1983-03-01), Sato et al.

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