Process for obtaining continuous lines of tumoral cells in vitro

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C12B 300, C12K 900

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040173615

ABSTRACT:
Process for obtaining cultures of tumoral cells in vitro, of specifically human origin.
The cultures of tumoral cells in vitro are prepared, particularly from human tumoral cells obtained from surgical samples of malignant tumors, by introducing, into the culture system, an anti-human lymphocyte serum described in the U. S. patent application Ser. No. 440,726, filed Feb. 8, 1974, which neutralizes the cytocidic and macrophagic activities of the lymphoblastoid and of the activated monocyte cells, present in the cellular system, with respect to the tumoral cells, thus permitting the establishment of continuous lines of tumoral cells in vitro.
Utilization of the continuous lines of tumoral cells in vitro thus obtained as means of elucidating the cancer process in man and as means of ascertaining the activity of new cancerostatic and cancerolytic medicaments.

REFERENCES:
willmer--Cells and Tissues in Culture vol. 3 (1966) pp. 343-345.

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