Process for obtaining an antigenic reagent useful for the indire

Details Process for obtaining an antigenic reagent useful for the indire Process for obtaining an antigenic reagent useful for the indire

1991-03-21

1995-04-11

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435244, 435245, 4352521, 435879, C12M 100, C12M 138, C12M 120

Patent

active

054057540

ABSTRACT:
The invention described herein consists of a process for preparing an antigenic reagent useful for the indirect determination of Salmonella typhi, the organism that is the causal agent of typhoid fever (TF). The invention consists on the following steps: to grow Salmonella typhi in a culture medium, characterized by containing a free-iron chelator, which generates a specific S. typhi outer membrane protein (OMP) pattern, OMPs that are used as a selective antigen for the detection of specific serum antibodies, by an immunoassay technique (ELISA).

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