Process for obtaining an active product from mammalian macrophag

Details Process for obtaining an active product from mammalian macrophag Process for obtaining an active product from mammalian macrophag

1976-03-02

1977-12-20

Rosen, Sam

Textiles: fiber preparation

424 95, C12K 900, A61K 3512

Patent

active

040640078

ABSTRACT:
Continuous cell lines of macrophages are provided by the in vitro action, on mammalian macrophages withdrawn in situ, of macrophage activating substances, which cause the removal by the macrophages of the other cells present in the cellular medium withdrawn. The macrophages thus isolated are then placed in a suitable culture medium, until the establishment of a continuous line of macrophages, then the macrophages established as a continuous line are cultured in the presence of a suitable activating substance and the supernatant liquid is collected from these cultures. This supernatant contains substances inhibiting the proliferation of the cells having a rapid rate of multiplication. This supernatant liquid is, if necessary, subjected to a purification process. The product is notably useful for inhibiting human tumor cells.

REFERENCES:
willmer -- Cells and Tissues in Culture- vol. 3 -- (1966) pp. 324-326.

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